Description: Rabbit polyclonal antibody to Focal Adhesion Kinase (Phospho-S843) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S843 of human Focal Adhesion Kinase protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PTK2 Alternative Names: FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAKEntrez Gene (Human): 5747Entrez Gene (Mouse) : 14083Entrez Gene (Rat) : 25614SwissProt (Human): Q05397SwissProt (Mouse) : P34152SwissProt (Rat) : O35346Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Focal Adhesion Kinase (Phospho-S843) expression in A549 (A), HepG2 (B), Hela (C), NIH3T3 (D), H9C2 (E) whole cell lysates.
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  Immunohistochemical analysis of Focal Adhesion Kinase (Phospho-S843) staining in human stomach cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.