Description: Rabbit polyclonal antibody to JAK2 (Phospho-Y221) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y221 of human JAK2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: JAK2 Alternative Names: Tyrosine-protein kinase JAK2; Janus kinase 2; JAK-2Entrez Gene (Human): 3717Entrez Gene (Mouse) : 16452Entrez Gene (Rat) : 24514SwissProt (Human): O60674SwissProt (Mouse) : Q62120SwissProt (Rat) : Q62689Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of JAK2 (Phospho-Y221) expression in HepG2 (A), LO2 (B) whole cell lysates.
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  Immunohistochemical analysis of JAK2 (Phospho-Y221) staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Direct ELISA antibody dose-response curve using Anti-JAK2 (Phospho-Y221) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.