Description: Rabbit polyclonal antibody to Topoisomerase 2 alpha (Phospho-S1106) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S1106 of human Topoisomerase 2 alpha protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: TOP2A Alternative Names: TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II alpha isozymeEntrez Gene (Human): 7153Entrez Gene (Mouse) : 21973Entrez Gene (Rat) : 360243SwissProt (Human): P11388SwissProt (Mouse) : Q01320SwissProt (Rat) : P41516Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Topoisomerase 2 alpha (Phospho-S1106) expression in Myla2059 (A), HuT78 (B), mouse testis (C), rat testis (D) whole cell lysates.
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  Immunohistochemical analysis of Topoisomerase 2 alpha (Phospho-S1106) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.