Description: Rabbit polyclonal antibody to HMGN2 (Phospho-S29) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S29 of human HMGN2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: HMGN2 Alternative Names: HMG17; Non-histone chromosomal protein HMG-17; High mobility group nucleosome-binding domain-containing protein 2Entrez Gene (Human): 3151Entrez Gene (Mouse) : 15331SwissProt (Human): P05204SwissProt (Mouse) : P09602SwissProt (Rat) : P18437Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HMGN2 (Phospho-S29) expression in rat lung (A) whole cell lysates.
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Immunohistochemical analysis of HMGN2 (Phospho-S29) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.