Description: Rabbit polyclonal antibody to VEGFR2/3 (Phospho-Y1054/Y1063) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1054/Y1063 of human VEGFR2/3 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: KDR; FLT4 Alternative Names: KDR; FLK1; VEGFR2; Vascular endothelial growth factor receptor 2; VEGFR-2; Fetal liver kinase 1; FLK-1; Kinase insert domain receptor; KDR; Protein-tyrosine kinase receptor flk-1; CD309; FLT4; VEGFR3; Vascular endothelial growth factor receptor 3; VEGFR-3; Fms-like tyrosine kinase 4; FLT-4; Tyrosine-protein kinase receptor FLT4Entrez Gene (Human): 3791; 2324Entrez Gene (Mouse) : 14257Entrez Gene (Rat) : 25589; 114110SwissProt (Human): P35968; P35916SwissProt (Mouse) : P35918; P35917SwissProt (Rat) : O08775; Q91ZT1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of VEGFR2/3 (Phospho-Y1054/Y1063) expression in AML12 (A), PMVEC (B), rat brain (C) whole cell lysates.
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  Immunohistochemical analysis of VEGFR2/3 (Phospho-Y1054/Y1063) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.