Description: Rabbit polyclonal antibody to L1CAM (Phospho-S1181) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S1181 of human L1CAM protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: L1CAM Alternative Names: CAML1; MIC5; Neural cell adhesion molecule L1; N-CAM-L1; NCAM-L1; CD171Entrez Gene (Human): 3897Entrez Gene (Rat) : 50687SwissProt (Human): P32004SwissProt (Mouse) : P11627SwissProt (Rat) : Q05695Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of L1CAM (Phospho-S1181) expression in Myla2059 (A) whole cell lysates.
-
Immunohistochemical analysis of L1CAM (Phospho-S1181) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.