Description: Rabbit polyclonal antibody to Caspase 9 (Phospho-S144) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S144 of human Caspase 9 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CASP9 Alternative Names: MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6Entrez Gene (Human): 842Entrez Gene (Mouse) : 12371SwissProt (Human): P55211SwissProt (Mouse) : Q8C3Q9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Caspase 9 (Phospho-S144) expression in HEK293T (A), HepG2 (B), A375 (C) whole cell lysates.
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  Immunohistochemical analysis of Caspase 9 (Phospho-S144) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.