Description: Rabbit polyclonal antibody to PKC mu (Phospho-S205) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S205 of human PKC mu protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: PRKD1 Alternative Names: PKD; PKD1; PRKCM; Serine/threonine-protein kinase D1; Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-muEntrez Gene (Human): 5587Entrez Gene (Mouse) : 18760Entrez Gene (Rat) : 85421SwissProt (Human): Q15139SwissProt (Mouse) : Q62101SwissProt (Rat) : Q9WTQ1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PKC mu (Phospho-S205) expression in HEK293T-PMA 10min (A), HEK293T-PMA 30min (B), H9C2 (C), NIH3T3 (D) whole cell lysates.
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Immunohistochemical analysis of PKC mu (Phospho-S205) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.