Description: Rabbit polyclonal antibody to Androgen Receptor (Phospho-S94) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S94 of human Androgen Receptor protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: AR Alternative Names: DHTR; NR3C4; Androgen receptor; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4Entrez Gene (Human): 367Entrez Gene (Mouse) : 11835Entrez Gene (Rat) : 24208SwissProt (Human): P10275SwissProt (Mouse) : P19091SwissProt (Rat) : P15207Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Androgen Receptor (Phospho-S94) expression in mouse muscle (A) whole cell lysates.
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Immunofluorescent analysis of Androgen Receptor (Phospho-S94) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.