Description: Rabbit polyclonal antibody to 53BP1 (Phospho-S6) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S6 of human 53BP1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: TP53BP1 Alternative Names: Tumor suppressor p53-binding protein 1; 53BP1; p53-binding protein 1; p53BP1Entrez Gene (Human): 7158SwissProt (Human): Q12888SwissProt (Mouse) : P70399Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of 53BP1 (Phospho-S6) expression in Hela (A), U87MG (B), 3T3L1 (C), H9C2 (D) whole cell lysates.
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  Immunohistochemical analysis of 53BP1 (Phospho-S6) staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.