Description: Rabbit polyclonal antibody to Thymidine Kinase 1 (Phospho-S13) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S13 of human Thymidine Kinase 1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: TK1 Alternative Names: Thymidine kinase cytosolicEntrez Gene (Human): 7083Entrez Gene (Mouse) : 21877Entrez Gene (Rat) : 24834SwissProt (Human): P04183SwissProt (Mouse) : P04184SwissProt (Rat) : P27158Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Thymidine Kinase 1 (Phospho-S13) expression in A375 (A), HCT116 (B), Myla2059 (C), Jurkat (D) whole cell lysates.
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  Immunohistochemical analysis of Thymidine Kinase 1 (Phospho-S13) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.