Description: Rabbit polyclonal antibody to MYOD1 (Phospho-S200) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S200 of human MYOD1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: MYOD1 Alternative Names: BHLHC1; MYF3; MYOD; Myoblast determination protein 1; Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3Entrez Gene (Human): 4654Entrez Gene (Mouse) : 17927Entrez Gene (Rat) : 337868SwissProt (Human): P15172SwissProt (Mouse) : P10085SwissProt (Rat) : Q02346Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MYOD1 (Phospho-S200) expression in EC9706 (A), mouse muscle (B) whole cell lysates.
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  Immunohistochemical analysis of MYOD1 (Phospho-S200) staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.