Description: Rabbit polyclonal antibody to FOXO1/3 (Phospho-S322+S325/pS318+S321) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S322+S325/pS318+S321 of human FOXO1/3 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: FOXO1; FOXO3 Alternative Names: FOXO1; FKHR; FOXO1A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; FOXO3; FKHRL1; FOXO3A; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1Entrez Gene (Human): 2308Entrez Gene (Mouse) : 56458; 56484Entrez Gene (Rat) : 84482SwissProt (Human): Q12778; O43524SwissProt (Mouse) : Q9R1E0; Q9WVH4SwissProt (Rat) : G3V7R4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of FOXO1/3 (Phospho-S322+S325/pS318+S321) expression in Beas2B (A), A549 (B), HepG2 (C) whole cell lysates.
• 
  Immunohistochemical analysis of FOXO1/3 (Phospho-S322+S325/pS318+S321) staining in human thyroid gland formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.