Description: Rabbit polyclonal antibody to RUNX1 (Phospho-S249) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S249 of human RUNX1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: RUNX1 Alternative Names: AML1; CBFA2; Runt-related transcription factor 1; Acute myeloid leukemia 1 protein; Core-binding factor subunit alpha-2; CBF-alpha-2; Oncogene AML-1; Polyomavirus enhancer-binding protein 2 alpha B subunit; PEA2-alpha B; PEBP2-alpha B; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunitEntrez Gene (Human): 861Entrez Gene (Rat) : 50662SwissProt (Human): Q01196SwissProt (Mouse) : Q03347SwissProt (Rat) : Q63046Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RUNX1 (Phospho-S249) expression in Jurkat (A) whole cell lysates.
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  Immunohistochemical analysis of RUNX1 (Phospho-S249) staining in human stomach cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.