Description: Rabbit polyclonal antibody to Histone H1oo (Acetyl-K163) Immunogen: KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K163 of human Histone H1oo protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: H1FOO Alternative Names: H1OO; OSH1; Histone H1oo; Oocyte-specific histone H1; Oocyte-specific linker histone H1; osH1Entrez Gene (Human): 132243Entrez Gene (Mouse) : 171506SwissProt (Human): Q8IZA3SwissProt (Mouse) : Q8VIK3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Histone H1oo (Acetyl-K163) expression in A2780 (A), U87MG (B), mouse testis (C), rat brain (D) whole cell lysates.
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  Immunohistochemical analysis of Histone H1oo (Acetyl-K163) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.