Description: Rabbit polyclonal antibody to CD47 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD47. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CD47 Alternative Names: MER6; Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD47Entrez Gene (Human): 961Entrez Gene (Mouse) : 16423Entrez Gene (Rat) : 29364SwissProt (Human): Q08722SwissProt (Mouse) : Q61735SwissProt (Rat) : P97829Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD47 expression in rat testis (A), mouse kindey (B), rat brain (C), mouse brain (D) whole cell lysates.
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  Immunohistochemical analysis of CD47 staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.