Description: Rabbit polyclonal antibody to MYO10 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MYO10. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: MYO10 Alternative Names: KIAA0799; Unconventional myosin-X; Unconventional myosin-10Entrez Gene (Human): 4651Entrez Gene (Mouse) : 17909Entrez Gene (Rat) : 310178SwissProt (Human): Q9HD67SwissProt (Mouse) : F8VQB6SwissProt (Rat) : D3ZJP6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MYO10 expression in Hela (A), A2780 (B), C6 (C), NIH3T3 (D) whole cell lysates.
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Immunohistochemical analysis of MYO10 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.