Description: Rabbit polyclonal antibody to AMPK gamma 1/2/3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AMPK gamma 1/2/3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: PRKAG1; PRKAG2; PRKAG3 Alternative Names: PRKAG1; 5'-AMP-activated protein kinase subunit gamma-1; AMPK gamma1; AMPK subunit gamma-1; AMPKg; PRKAG2; 5'-AMP-activated protein kinase subunit gamma-2; AMPK gamma2; AMPK subunit gamma-2; H91620p; PRKAG3; AMPKG3; 5'-AMP-activated protein kinase subunit gamma-3; AMPK gamma3; AMPK subunit gamma-3Entrez Gene (Human): 5571; 51422; 53632;Entrez Gene (Mouse) : 19082; 108099; 241113Entrez Gene (Rat) : 25520SwissProt (Human): P54619; Q9UGJ0; Q9UGI9SwissProt (Mouse) : O54950; Q91WG5; Q8BGM7SwissProt (Rat) : P80385Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of AMPK gamma 1/2/3 expression in HEK293T (A), HepG2 (B), MCF7 (C), PC3 (D) whole cell lysates.
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  Immunofluorescent analysis of AMPK gamma 1/2/3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.