Description: Rabbit polyclonal antibody to PKC gamma (Phospho-T514) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T514 of human PKC gamma protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PRKCG Alternative Names: PKCG; Protein kinase C gamma type; PKC-gammaEntrez Gene (Human): 5582Entrez Gene (Rat) : 24681SwissProt (Human): P05129SwissProt (Rat) : P63319Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PKC gamma (Phospho-T514) expression in rat brain (A) whole cell lysates.
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  Immunohistochemical analysis of PKC gamma (Phospho-T514) staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Direct ELISA antibody dose-response curve using Anti-PKC gamma (Phospho-T514) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.