Description: Rabbit polyclonal antibody to PTP1B (Phospho-Y66) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y66 of human PTP1B protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000) Gene Symbol: PTPN1 Alternative Names: PTP1B; Tyrosine-protein phosphatase non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP-1BEntrez Gene (Human): 5770Entrez Gene (Mouse) : 19246Entrez Gene (Rat) : 24697SwissProt (Human): P18031SwissProt (Mouse) : P35821SwissProt (Rat) : P20417Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PTP1B (Phospho-Y66) expression in MCF7 (A), A549 (B), mouse lung (C) whole cell lysates.
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Direct ELISA antibody dose-response curve using Anti-PTP1B (Phospho-Y66) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.