Description: Rabbit polyclonal antibody to MBP (Phospho-T232) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T232 of human MBP protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: MBP Alternative Names: Myelin basic protein; MBP; Myelin A1 protein; Myelin membrane encephalitogenic proteinEntrez Gene (Human): 4155Entrez Gene (Mouse) : 17196Entrez Gene (Rat) : 24547SwissProt (Human): P02686SwissProt (Mouse) : P04370SwissProt (Rat) : P02688Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MBP (Phospho-T232) expression in mouse brain (A), rat brain (B) whole cell lysates.
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  Immunohistochemical analysis of MBP (Phospho-T232) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Direct ELISA antibody dose-response curve using Anti-MBP (Phospho-T232) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.