Description: Rabbit polyclonal antibody to MARCKS (Phospho-S158) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S158 of human MARCKS protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: MARCKS Alternative Names: MACS; PRKCSL; Myristoylated alanine-rich C-kinase substrate; MARCKS; Protein kinase C substrate 80 kDa protein light chain; 80K-L protein; PKCSLEntrez Gene (Human): 4082Entrez Gene (Mouse) : 17118SwissProt (Human): P29966SwissProt (Mouse) : P26645SwissProt (Rat) : P30009Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MARCKS (Phospho-S158) expression in mouse kidney (A), rat brain (B) whole cell lysates.
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  Immunohistochemical analysis of MARCKS (Phospho-S158) staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.