Description: Rabbit polyclonal antibody to PINK1 (Phospho-S228) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S228 of human PINK1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PINK1 Alternative Names: Serine/threonine-protein kinase PINK1 mitochondrial; BRPK; PTEN-induced putative kinase protein 1Entrez Gene (Human): 65018Entrez Gene (Mouse) : 68943SwissProt (Human): Q9BXM7SwissProt (Mouse) : Q99MQ3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PINK1 (Phospho-S228) expression in mouse testis (A), rat testis (B), HEK293T (C), MCF7 (D), U87MG (E) whole cell lysates.
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  Immunohistochemical analysis of PINK1 (Phospho-S228) staining in human tonsil cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Direct ELISA antibody dose-response curve using Anti-PINK1 (Phospho-S228) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.