Description: Rabbit polyclonal antibody to Ubiquitin (Phospho-S65) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S65 of human Ubiquitin protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000) Gene Symbol: UBA52; RPS27A; UBB; UBC Alternative Names: UBB; Polyubiquitin-B; UBC; Polyubiquitin-C; RPS27A; UBA80; UBCEP1; Ubiquitin-40S ribosomal protein S27a; Ubiquitin carboxyl extension protein 80; UBA52; UBCEP2; Ubiquitin-60S ribosomal protein L40; CEP52; Ubiquitin A-52 residue ribosomal protein fusion product 1Entrez Gene (Human): 7314; 7316; 6233; 7311SwissProt (Human): P0CG47; P0CG48; P62979; P62987Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Ubiquitin (Phospho-S65) expression in C6 (A), AML12 (B), SGC7901-UV treated (C), SGC7901 (D) whole cell lysates.
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  Direct ELISA antibody dose-response curve using Anti-Ubiquitin (Phospho-S65) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.