Description: Rabbit polyclonal antibody to CDK9 (Phospho-T186) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T186 of human CDK9 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CDK9 Alternative Names: CDC2L4; TAK; Cyclin-dependent kinase 9; C-2K; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunitEntrez Gene (Human): 1025Entrez Gene (Mouse) : 107951Entrez Gene (Rat) : 362110SwissProt (Human): P50750SwissProt (Mouse) : Q99J95SwissProt (Rat) : Q641Z4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CDK9 (Phospho-T186) expression in PC3 (A), mouse liver (B), rat liver (C) whole cell lysates.
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  Immunohistochemical analysis of CDK9 (Phospho-T186) staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.