Description: Rabbit polyclonal antibody to CDK7 (Phospho-T170) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T170 of human CDK7 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CDK7 Alternative Names: CAK; CAK1; CDKN7; MO15; STK1; Cyclin-dependent kinase 7; 39 kDa protein kinase; p39 Mo15; CDK-activating kinase 1; Cell division protein kinase 7; Serine/threonine-protein kinase 1; TFIIH basal transcription factor complex kinase subunitEntrez Gene (Human): 1022Entrez Gene (Mouse) : 12572SwissProt (Human): P50613SwissProt (Mouse) : Q03147Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CDK7 (Phospho-T170) expression in Hela (A), HEK293T (B), A549 (C), MCF7 (D), Raw264.7 (E), mouse spleen (F) whole cell lysates.
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Immunohistochemical analysis of CDK7 (Phospho-T170) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.