Description: Rabbit polyclonal antibody to RSK1 (Phospho-T359/S363) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T359/S363 of human RSK1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: RPS6KA1 Alternative Names: MAPKAPK1A; RSK1; Ribosomal protein S6 kinase alpha-1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; Ribosomal S6 kinase 1; RSK-1Entrez Gene (Human): 6195Entrez Gene (Mouse) : 20111Entrez Gene (Rat) : 81771SwissProt (Human): Q15418SwissProt (Mouse) : P18653SwissProt (Rat) : Q63531Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RSK1 (Phospho-T359/S363) expression in PC3 (A) whole cell lysates.
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  Immunohistochemical analysis of RSK1 (Phospho-T359/S363) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.