Description: Rabbit polyclonal antibody to Glucocorticoid Receptor (Phospho-S226) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S226 of human Glucocorticoid Receptor protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: NR3C1 Alternative Names: GRL; Glucocorticoid receptor; GR; Nuclear receptor subfamily 3 group C member 1Entrez Gene (Human): 2908Entrez Gene (Rat) : 24413SwissProt (Human): P04150SwissProt (Mouse) : P06537SwissProt (Rat) : P06536Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Glucocorticoid Receptor (Phospho-S226) expression in A549 (A), Hela (B), U87MG (C), PC12 (D) whole cell lysates.
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  Immunohistochemical analysis of Glucocorticoid Receptor (Phospho-S226) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.