Description: Rabbit polyclonal antibody to C/EBP alpha (Phospho-S21) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S21 of human C/EBP alpha protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CEBPA Alternative Names: CCAAT/enhancer-binding protein alpha; C/EBP alphaEntrez Gene (Human): 1050Entrez Gene (Mouse) : 12606Entrez Gene (Rat) : 24252SwissProt (Human): P49715SwissProt (Mouse) : P53566SwissProt (Rat) : P05554Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of C/EBP alpha (Phospho-S21) expression in rat lung (A) whole cell lysates.
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Immunohistochemical analysis of C/EBP alpha (Phospho-S21) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.