Description: Rabbit polyclonal antibody to Mast Cell Tryptase Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Mast Cell Tryptase. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: TPSAB1; TPSB2 Alternative Names: TPSAB1; TPS1; TPS2; TPSB1; Tryptase alpha/beta-1; Tryptase-1; Tryptase I; Tryptase alpha-1; TPSB2; TPS2; Tryptase beta-2; Tryptase-2; Tryptase IIEntrez Gene (Human): 7177Entrez Gene (Mouse) : 100503895Entrez Gene (Rat) : 54271; 29268SwissProt (Human): Q15661; P20231SwissProt (Mouse) : Q02844; P21845SwissProt (Rat) : P27435; P50343Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Mast Cell Tryptase expression in MCF7 (A), mouse heart (B), PMVEC (C), A375 (D) whole cell lysates.
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  Immunohistochemical analysis of Mast Cell Tryptase staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.