Description: Rabbit polyclonal antibody to Actin-pan Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Actin-pan. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: ACTA2 Alternative Names: ACTA2; ACTSA; ACTVS; GIG46; Actin aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 proteinEntrez Gene (Human): 59Entrez Gene (Mouse) : 11475Entrez Gene (Rat) : 81633SwissProt (Human): P62736; P60709; P68032; P63261; P63267; P68133; Q562R1SwissProt (Mouse) : P62737SwissProt (Rat) : P62738Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Actin-pan expression in Hela (A), mouse kidney (B), rat ovary (C), HEK293T (D), A2780 (E) whole cell lysates.
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  Immunohistochemical analysis of Actin-pan staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.