Description: Rabbit polyclonal antibody to GPR48 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR48. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: LGR4 Alternative Names: GPR48; Leucine-rich repeat-containing G-protein coupled receptor 4; G-protein coupled receptor 48Entrez Gene (Human): 55366Entrez Gene (Mouse) : 107515Entrez Gene (Rat) : 286994SwissProt (Human): Q9BXB1SwissProt (Mouse) : A2ARI4SwissProt (Rat) : Q9Z2H4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GPR48 expression in HEK293T (A), PC3 (B), SP20 (C), PC12 (D) whole cell lysates.
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Immunofluorescent analysis of GPR48 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.