Description: Rabbit polyclonal antibody to CD97 alpha Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD97 alpha. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CD97 Alternative Names: CD97 antigen; Leukocyte antigen CD97; CD97Entrez Gene (Human): 976SwissProt (Human): P48960Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD97 alpha expression in Jurkat (A), HCT116 (B), H9C2 (C) whole cell lysates.
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Immunohistochemical analysis of CD97 alpha staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.