Description: Rabbit polyclonal antibody to ADAM10 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ADAM10. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: ADAM10 Alternative Names: KUZ; MADM; Disintegrin and metalloproteinase domain-containing protein 10; ADAM 10; CDw156; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease; CD156cEntrez Gene (Human): 102Entrez Gene (Mouse) : 11487SwissProt (Human): O14672SwissProt (Mouse) : O35598SwissProt (Rat) : Q10743Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ADAM10 expression in HepG2 (A), U87MG (B), A549 (C), SP20 (D), PC12 (E) whole cell lysates.
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  Immunohistochemical analysis of ADAM10 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.