Description: Rabbit polyclonal antibody to Factor II Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Factor II. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: F2 Alternative Names: Prothrombin; Coagulation factor IIEntrez Gene (Human): 2147Entrez Gene (Mouse) : 14061SwissProt (Human): P00734SwissProt (Mouse) : P19221SwissProt (Rat) : P18292Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Factor II expression in K562 (A), A549 (B), LO2 (C), rat liver (D), mouse liver (E) whole cell lysates.
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Immunohistochemical analysis of Factor II staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.