Description: Rabbit polyclonal antibody to Desmoglein 1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Desmoglein 1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: DSG1 Alternative Names: CDHF4; Desmoglein-1; Cadherin family member 4; Desmosomal glycoprotein 1; DG1; DGI; Pemphigus foliaceus antigenEntrez Gene (Human): 1828SwissProt (Human): Q02413Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Desmoglein 1 expression in Beas2B (A), H9C2 (B), MEF (C) whole cell lysates.
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Immunohistochemical analysis of Desmoglein 1 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.