Description: Rabbit polyclonal antibody to GNE Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GNE. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: GNE Alternative Names: GLCNE; Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinaseEntrez Gene (Human): 10020Entrez Gene (Mouse) : 50798Entrez Gene (Rat) : 114711SwissProt (Human): Q9Y223SwissProt (Mouse) : Q91WG8SwissProt (Rat) : O35826Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GNE expression in LO2 (A), mouse liver (B), rat liver (C) whole cell lysates.
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  Immunohistochemical analysis of GNE staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.