Description: Rabbit polyclonal antibody to ZNF575 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ZNF575. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: ZNF575 Alternative Names: Zinc finger protein 575Entrez Gene (Human): 284346Entrez Gene (Mouse) : 101544SwissProt (Human): Q86XF7SwissProt (Mouse) : Q3TXZ1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ZNF575 expression in Hela (A), CT26 (B), AML12 (C), C6 (D), H9C2 (E), MEF (F) whole cell lysates.
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Immunohistochemical analysis of ZNF575 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.