Description: Rabbit polyclonal antibody to PTPN22 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human PTPN22. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PTPN22 Alternative Names: PTPN8; Tyrosine-protein phosphatase non-receptor type 22; Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP; Lymphoid phosphatase; LyP; PEST-domain phosphatase; PEPEntrez Gene (Human): 26191Entrez Gene (Mouse) : 19260SwissProt (Human): Q9Y2R2SwissProt (Mouse) : P29352Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PTPN22 expression in Jurkat (A), K562 (B), C6 (C), BV2 (D) whole cell lysates.
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Immunohistochemical analysis of PTPN22 staining in human aratracheal lymph nodes formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.