Description: Rabbit polyclonal antibody to SLY1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SLY1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: SCFD1 Alternative Names: C14orf163; KIAA0917; STXBP1L2; Sec1 family domain-containing protein 1; SLY1 homolog; Sly1p; Syntaxin-binding protein 1-like 2Entrez Gene (Human): 23256Entrez Gene (Mouse) : 76983Entrez Gene (Rat) : 54350SwissProt (Human): Q8WVM8SwissProt (Mouse) : Q8BRF7SwissProt (Rat) : Q62991Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SLY1 expression in CT26 (A), PC12 (B), LO2 (C), HEK293T (D) whole cell lysates.
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Immunohistochemical analysis of SLY1 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.