Description: Rabbit polyclonal antibody to ARHGEF12 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ARHGEF12. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: ARHGEF12 Alternative Names: KIAA0382; LARG; Rho guanine nucleotide exchange factor 12; Leukemia-associated RhoGEFEntrez Gene (Human): 23365Entrez Gene (Mouse) : 69632SwissProt (Human): Q9NZN5SwissProt (Mouse) : Q8R4H2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of ARHGEF12 expression in A549 (A), LO2 (B), HEK293T (C), CT26 (D), PC12 (E) whole cell lysates.
-
Immunohistochemical analysis of ARHGEF12 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.