Description: Rabbit polyclonal antibody to RGS14 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RGS14. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: RGS14 Alternative Names: Regulator of G-protein signaling 14; RGS14Entrez Gene (Human): 10636Entrez Gene (Mouse) : 51791Entrez Gene (Rat) : 114705SwissProt (Human): O43566SwissProt (Mouse) : P97492SwissProt (Rat) : O08773Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RGS14 expression in LOVO (A), BV2 (B), PC12 (C) whole cell lysates.
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Immunohistochemical analysis of RGS14 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.