Description: Rabbit polyclonal antibody to PPM1L Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PPM1L. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PPM1L Alternative Names: PP2CE; Protein phosphatase 1L; Protein phosphatase 1-like; Protein phosphatase 2C isoform epsilon; PP2C-epsilonEntrez Gene (Human): 151742Entrez Gene (Mouse) : 242083SwissProt (Human): Q5SGD2SwissProt (Mouse) : Q8BHN0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PPM1L expression in AML12 (A), H1792 (B), COS7 (C) whole cell lysates.
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Immunohistochemical analysis of PPM1L staining in human prostate formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.