Description: Rabbit polyclonal antibody to MYPT3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MYPT3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PPP1R16A Alternative Names: MYPT3; Protein phosphatase 1 regulatory subunit 16A; Myosin phosphatase-targeting subunit 3Entrez Gene (Human): 84988Entrez Gene (Mouse) : 73062SwissProt (Human): Q96I34SwissProt (Mouse) : Q923M0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MYPT3 expression in HEK293T (A), A2780 (B), HCT116 (C), H9C2 (D), CT26 (E) whole cell lysates.
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Immunohistochemical analysis of MYPT3 staining in human prostate formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.