Description: Rabbit polyclonal antibody to Gemin3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Gemin3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: DDX20 Alternative Names: DP103; GEMIN3; Probable ATP-dependent RNA helicase DDX20; Component of gems 3; DEAD box protein 20; DEAD box protein DP 103; Gemin-3Entrez Gene (Human): 11218Entrez Gene (Mouse) : 53975SwissProt (Human): Q9UHI6SwissProt (Mouse) : Q9JJY4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Gemin3 expression in A549 (A), HCC827 (B), H9C2 (C), CT26 (D) whole cell lysates.
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  Immunohistochemical analysis of Gemin3 staining in human tonsil cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.