Description: Rabbit polyclonal antibody to p46 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human p46. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: POLDIP3 Alternative Names: KIAA1649; PDIP46; Polymerase delta-interacting protein 3; 46 kDa DNA polymerase delta interaction protein; p46; S6K1 Aly/REF-like target; SKAREntrez Gene (Human): 84271Entrez Gene (Mouse) : 73826SwissProt (Human): Q9BY77SwissProt (Mouse) : Q8BG81Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of p46 expression in PC12 (A), CT26 (B), Hela (C), MCF7 (D) whole cell lysates.
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Immunohistochemical analysis of p46 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.