Description: Rabbit polyclonal antibody to Nectin 4 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Nectin 4. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PVRL4 Alternative Names: LNIR; PRR4; Poliovirus receptor-related protein 4; Ig superfamily receptor LNIR; Nectin-4Entrez Gene (Human): 81607Entrez Gene (Mouse) : 71740SwissProt (Human): Q96NY8SwissProt (Mouse) : Q8R007Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Nectin 4 expression in Hela (A), MCF7 (B), MEF (C), PC12 (D) whole cell lysates.
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Immunohistochemical analysis of Nectin 4 staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.