Description: Rabbit polyclonal antibody to USP50 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human USP50. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: USP50 Alternative Names: Inactive ubiquitin carboxyl-terminal hydrolase 50; Inactive ubiquitin-specific peptidase 50Entrez Gene (Human): 373509Entrez Gene (Mouse) : 75083SwissProt (Human): Q70EL3SwissProt (Mouse) : Q6P8X6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of USP50 expression in MCF7 (A), Hela (B), PC12 (C), CT26 (D) whole cell lysates.
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  Immunohistochemical analysis of USP50 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.