Description: Rabbit polyclonal antibody to EIF2AK1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EIF2AK1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: EIF2AK1 Alternative Names: HRI; KIAA1369; Eukaryotic translation initiation factor 2-alpha kinase 1; Heme-controlled repressor; HCR; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; Hemin-sensitive initiation factor 2-alpha kinaseEntrez Gene (Human): 27102Entrez Gene (Mouse) : 15467Entrez Gene (Rat) : 27137SwissProt (Human): Q9BQI3SwissProt (Mouse) : Q9Z2R9SwissProt (Rat) : Q63185Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of EIF2AK1 expression in K562 (A), LO2 (B), PC12 (C), AML12 (D) whole cell lysates.
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  Immunohistochemical analysis of EIF2AK1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.