Description: Rabbit polyclonal antibody to RNF144B Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RNF144B. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: RNF144B Alternative Names: IBRDC2; P53RFP; E3 ubiquitin-protein ligase RNF144B; IBR domain-containing protein 2; RING finger protein 144B; p53-inducible RING finger proteinEntrez Gene (Human): 255488Entrez Gene (Mouse) : 218215SwissProt (Human): Q7Z419SwissProt (Mouse) : Q8BKD6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RNF144B expression in H1792 (A), A549 (B), CT26 (C), PC12 (D) whole cell lysates.
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Immunohistochemical analysis of RNF144B staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.